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We assessed the potential impact of the remaining sample “contamination” with soil on overall FGCM excretion patterns by determining in the samples from 2013 (n = 176 samples from 10 ACTH challenges, see “ACTH challenge” in Methods section) the amount of soil and subsequently comparing between fecal hormone concentrations (for both the CCST and 11β-hydroxyetiocholanolone EIA assays) uncorrected and corrected for soil content, i.e. expressed per fecal mass without soil (see [67]).
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