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  • The membrane was blocked with 5% BSA in 0.05% TBST for 1 h at room temperature and then immunoblotted with the following antibodies: anti-β-actin (#3700, 1:1000, Cell Signaling Technology, Danvers, USA), anti-NMDAR1 (#4204, 1:1000, Cell Signaling Technology, Danvers, USA), anti-NMDAR2B (#MAB5220, 1:1000, EMD Millipore Corporation, Billerica, USA) and anti-GR (#SAB4501310, 1:500, Sigma-Aldrich) in blocking buffer at 4°C overnight.
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