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  • The zygotes were then loaded for 45 min with 20 μM sodium-binding benzofuran isophthalate acetoxymethyl ester (SBFI-AM; Life Technologies, Grand Island, NY) in PBS alone, PBS containing 0.2% α-chymotrypsin, or PBS containing 0.2% α-chymotrypsin and a combination of α-defensin 1 and β-defensin 3 (Abcam, Cambridge, MA) each at 50 μg/mL.
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