DNA containing an acrosin signal sequence, EGFP, and Thy1 C-terminal GPI-anchoring sequence was amplified by PCR from the pCX::GFP-GPI2 construct, kindly provided by Anna-Katerina Hadjantonakis (Rhee et al., 2006), and cloned into the SalI and XbaI sites of the pIVT expression plasmid (Igarashi et al., 2007).
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