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  • An approximately 500 bp region of the 16S rRNA gene (covering V1–V3) was PCR-amplified from extracted DNA samples using composite fusion primers comprising universal 16 S primers (27FYM and 519R) along with Roche GS-FLX Titanium Series adapter sequences (A & B) for 454 pyrosequencing using the Lib-L emPCR method.
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