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  • Sub-culture and identification of colonies of interest in mixed cultures: After 8–28 days of incubation, culture plates were inspected under a dissecting microscope and any colonies of interest (namely microcolonies or colonies showing satellitism: growing either around or on larger colonies) were passaged onto: (a) fresh BA plates, cross-streaked with helper strains (Propionibacterium acnes ATCC 6919, Fusobacterium nucleatum subspecies polymorphum NCTC 10562, or colonies in proximity to the colonies of interest) and where relevant, supplemented with either pyoverdines-Fe or desferricoprogen; or (b) cellulose acetate membranes overlying lawn cultures of the helper strains.
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