ELISA assay for brain and mitochondrial Aβ Measurement: Mouse cortex or mitochondrial fractions were incubated in 5 M guanidine HCl and 50 mM Tris HCl (pH 8.0) overnight and then subjected to Aβ concentration detection using human Aβ1–40 and Aβ1–42 ELISA kits (Life technology) following the manufacturer’s instructions [24, 25].
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