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nif:broaderContext
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nif:isString
  • After blocking in TBS buffer (20 mM Tris-HCl, 150 mM sodium chloride) containing 5% (wt/vol) nonfat dry milk for 1 h at room temperature, the membrane was then incubated and gently shaken overnight (at 4°C) with primary antibodies.
rdf:type