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  • The standard curves (inter-assay reproducibility, n = 11) showed a mean square error (MSE) of ≤0.111, correlation coefficient (r2) of ≥0.998 and slopes of 3.28 (mean) ± 0.05 (standard deviation), indicating a high amplification efficiency (≥1.99) (2 would indicate 100% PCR efficiency).
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