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  • In order to analyze the distribution and load of Leishmania amastigotes within the cutaneous lesion, samples were collected from 3 different sites, in the following order: the center of the ulcer, the base (inner border) of the ulcer, and the raised border of the ulcer (Fig 1); using a randomly chosen coordinate defined as North, South, East or West, taking as reference the lateral and longitudinal axes of the human body.
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