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  • Membranes were then probed with primary antibodies diluted in 1-5% milk or BSA for 3 hrs at room temperature or overnight at 4°C: rabbit anti-DYNC2LI1 [26] (1:200; LIC3), rabbit anti-WDR34 (1:200; Abcam ab81030), rabbit anti-DYNC2H1 [26] (1:150; Dynein HC2), rabbit anti-GAPDH (1:1,000; 14C10; Cell Signaling Technology #2118), mouse anti-β-tubulin (1:10,000; GTU-88; Abcam ab11316), mouse anti-β-tubulin (1:5000; Sigma T9026), and goat anti-GLI3 (1 μg mL; R&D Systems AF3690).
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