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  • Cell lysates (300 μg) from T24 and 5637 BC cells, untreated or treated for 1 and 3 h with sorafenib (20 μM) or for 3 h with perifosine (0.5, 1 and 2.5 μM), were immunoprecipitated using anti-CB mAb following the Immunoprecipitation Kit Dynabeads® Protein A instructions (Life Technologies, Monza, Italy).
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