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  • The p53 mutant, 5637 and T24 BC cell lines, purchased from American Type Culture Collection (ATCC, Rockville, MD, USA), were maintained in RPMI-1640 medium (Lonza Bioresearch, Basel, Switzerland) supplemented with 10% heat-inactivated fetal bovine serum, 2.5 mM N-2-hydroxyethylpiperazine-N'-2- ethanesulfonicacid (HEPES), 2mM L-glutamine, 100 IU/ml of penicillin, 100 μg/ml of streptomycin (Lonza) at 37°C, 5% CO and 95% humidity.
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