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  • Each microsatellite locus was amplified separately using a GeneAmp PCR 9600 thermal-cycler (Perkin Elmer, Wellesley, Massachusetts) in a total volume of 10μl containing 1μl of 100–250 ng/μl purified DNA template, 0.5 μM/μl forward and reverse primer, 4μl PCR Mastermix 2X (Taq polymerase with manufacturer’s supplied buffer, dNTPs and MgCl2, Promega, Madison, Wisconsin), and 4μl DNA/RNA free dH2O.
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