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  • 0 (xsd:integer)
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  • 469 (xsd:integer)
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  • Multiplex ligation-dependent probe amplification (MLPA): The multiplex ligation-dependent probe amplification procedure, using the SALSA MS-MLPA kit ME042-B1 CIMP probe mix (for detecting methylation status in the promoter regions of 8 different genes; RUNX3, MLH1, NEUROG1, CDKN2A, IGF2, CRABP1, SOCS1 and CACNA1G) as given in Table 1, were performed according to the vendors recommendations (MRC-Holland, Amsterdam, the Netherlands), and as previously described [34].
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