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  • We represent reversible binding of AMPK to ULK1 using two unidirectional rules, as follows: AMPK(ulk1,T172~P)+ULK1(stampk,S758~0)-> \AMPK(ulk1!1,T172~P).ULK1(stampk!1,S758~0)a5(5A) AMPK(ulk1!1).ULK1(stampk!1)->AMPK(ulk1)+ULK1(stampk)d5(5B) The first rule, Equation (5A), indicates that AMPK, when phosphorylated at T172 and free of ULK1, is able to associate with ULK1, provided that ULK1 is not phosphorylated at S758.
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