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  • Chromatography conditions included column Synergi Hydro-RP C18 (Phenomenex, 4.6×250 mm); detection UV absorption at 230 nm; mobile phase: (A) methanol; (B) water; gradient elution (Time, A%): (0 min, 40%; 12 min, 75%; 26 min,75%; 30 min, 40%; 37 min, 40%) and flow rate was 1 ml/min; column temperature 30°; injection volume 20 μ1.
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