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The samples were then washed and labelled with appropriate secondary antibodies (Texas Red-conjugated donkey anti-goat antibody (1∶150) and DyLight-conjugated Donkey anti-mouse antibody (1∶400) [both from Jackson ImmunoResearch]; Alexa Fluor 488-conjugated donkey anti-rabbit antibody [1∶500, Molecular Probes]) for 1 hour at room temperature.
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