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  • The sections were incubated overnight at 4°C with goat anti-human SOX2 (1∶25 in PBS; Chemicon), alone or in combination with mouse anti-Ki67 (1∶250, Dako), mouse anti-GFAP (1∶200, Abcam), mouse anti-NeuN (1∶800), rabbit anti-Iba-1 (1∶200, Wako), or mouse anti-CNPase (1∶200, Sigma).
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