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  • The cells were washed, blocked with 5% goat serum in 0.1 M DPBS for 30 minutes at 4°C and incubated in primary antibody (A2B5 clone 105 hybridoma supernatant [ATCC, 1∶1]; anti O4 [Millipore, 1∶50]) for 45 minutes at 4°C.
rdf:type