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  • The cell suspension was then incubated in A2B5 hybridoma supernatant (ATCC) for 30 minutes at 4°C, washed with 0.1 M PBS, 0.5% bovine serum albumin three times, and finally incubated in a secondary antibody (Alexa Fluor 488 goat anti-mouse, 1∶500 in 0.1 M PBS [Invitrogen]) and propidium iodide.
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