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  • The dissociated cells were resuspended in defined proliferative media composed of DMEM-F12, non-essential amino acids (100 µm), HEPES (2 mM), D-glucose (30 mM), bovine serum albumin (0.001%), sodium pyruvate (1 mM), l-glutamine (2 mM), N2 supplement (1X), penicillin-streptomycin-fungizone (1X) (all from Gibco-Invitrogen); and hydrocortisone (300 ng/mL) and triiodothyronine (30 ng/mL) from Sigma.
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