Enzymatic digestion was sequentially performed with Solution I (Papain [14 U/mL, Sigma] and DNase I [10 U/mL, Sigma] in PIPES solution) for 90 minutes at 37°C and Solution II (Papain [7 U/mL] and DNase I [15 U/mL] in PIPES: proliferative media [1∶1]) for 30 minutes at 37°C.
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