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Levels of F4/80, IL-6, adiponectin, MCP-1, TNFα and IL-10 were measured by quantitative Real-Time PCR (qRT-PCR), which was carried out using the LightCycler 480 SYBR Green I Master Mix chemistry (Roche Diagnostics, Indianapolis, IN) and analyzed on the LightCycler 480 instrument (Roche Diagnostics, Indianapolis, IN).
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