Adipocytes were then co-cultured with isolated splenocytes from wild type C57Bl/6J, GFP-expressing C57Bl/6J or TNFα -/- mice in direct and indirect contact systems in the presence or absence of LPS (E. coli 0111:B4 – 1 µg/mL; Sigma Aldrich, St. Louis, MO) in complete DMEM.