lacZ Reporter Fusion and β-galactosidase Assay: The H3-T6SS left-lacZ and H3-T6SS right-lacZ transcriptional fusions were constructed by PCR amplification of respectively 486 and 494 bp upstream DNA region from the lip3 or hsiB3 gene by using TSO15/TSO16 and TSO17/TSO18 primers (Table 1).
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