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  • Determination of gh, ghr, igf, and β-actin mRNA Levels: The levels of gh, ghr, and igf1 mRNA expressions in the tissues were determined by real-time quantitative PCR (qPCR) with an equipment of ABI Prism 7000 Sequence Detection System (Applied Biosystems-Life Technologies, Foster City, CA) using β-actin as an internal standard according to Raven et al. [20].
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