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For D-dimer and fibrinogen assays, 2.7 ml of blood was taken from the antecubital vein of all patients, placed in standard Vacutainer (Becton Dickinson, Plymouth, UK) tubes containing 0.109 M buffered tri-sodium citrate, and centrifuged at 1 000 × g for 10 minutes at 18-21°C to extract the plasma.
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