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  • The hydrolytic activity of the remaining enzyme was quantified in a final concentration of 1 mM of the BApNA, Suc-AAPFpNA, Suc-AAPLpNA, Z-GGLpNA and BPVA-pNA (Sigma-Aldrich) chromogenic substrates for trypsin, α-chymotrypsin, elastase, subtilisin and thrombin (Sigma-Aldrich), respectively.
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