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  • Twenty microlitres of Sc-SRP-6 (3.5 µg/µl) was incubated with 10 µl of each enzyme (1 µg/µl) for 10 min at 37°C in microtiter plates with the total volume adjusted to 45 µl with 0.1 M Tris-HCl (pH 8) containing 0.1 M NaCl and 1 mM CaCl2.
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