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nif:beginIndex
  • 0 (xsd:integer)
nif:broaderContext
nif:endIndex
  • 282 (xsd:integer)
nif:isString
  • The membrane was blocked with 0.01 M Tris-HCl (pH 7.5) containing 0.1 M NaCl, 5% (w/v) BSA and 0.05% (v/v) Tween-20 for 30 min at room temperature (RT) and then incubated with peroxidase-conjugated mouse anti-His6-tag antibody diluted (1∶5,000) in blocking solution for 2 hrs at RT.
rdf:type