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  • The lysate was washed for 4 hrs in 1 L 0.05 M Tris-HCl (pH 7.5) containing 0.5 M NaCl (TN), followed by 4 hrs in TN containing 1% Triton X-100, and the pellet with inclusion bodies was recovered by centrifugation at 6,500×g for 20 min at 4°C.
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