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Serial sections of tumor xenografts from mice treated with placebo and combination treatment (gefitinib plus DIM-C) were incubated overnight at 4°C, with primary specific antibodies against PPARγ (sc-7273 mouse monoclonal IgG antibody 1∶1000 dilution, Santa Cruz, CA, USA), p21 (12D1 rabbit antibody 1∶100 dilution, cell signaling, MA, USA).
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