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  • Briefly, PCR amplification was carried out in reaction mixtures containing 35 μl of primer-nucleotide-mix (Hain Lifescience GmbH, Nehren, Germany), 5 μl 10x PCR buffer for HotStarTaq (Qiagen GmbH, Hilden, Germany), 2 μl 25 mM MgCl2 solution (Qiagen GmbH, Hilden, Germany), 0.2 μl HotStarTaq (Qiagen GmbH, Hilden, Germany), 3 μl H2O and 5 μl of DNA positively tested in the PCR assay.
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