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If PCR inhibition was observed (wild boar samples number TI132, TI133, TI134, TI135, TG413 and TG435; presumably due to tissue contaminants), the DNA samples were 5x diluted in lysis/elution buffer (v:v, 1:1) of the Roche respiratory DNA extraction kit (Roche Diagnostics, Basel, Switzerland) and reanalyzed using the COBAS® TaqMan® MTB Test kit.
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