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  • For mechanical disruption, 200 µl Tissue Lysis Buffer (Roche Diagnostics, Basel, Switzerland) was added to the pellet, and samples were homogenized twice using tubes containing ceramic beads (Omni International, Kennesaw, United States) and a Precellys 24 homogenizer (Bertin Technologies, Montigny, France) for 45 sec. at 6.500 rpm.
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