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  • Two modifications to the above mentioned protocol were made to enhance the chance for cultivation of slow-growing mycobacteria of the MTBC: Tissue material was decontaminated using N-Acetyl-L-Cystein-NaOH (NALC-NaOH) from the BD MycoPrep™ Specimen Digestion/Decontamination Kit (Becton, Dickinson and Company, Franklin Lakes, New Jersey, United States) instead of H2SO4 and NaOH [43] and the incubation time of liquid media was prolonged to twelve weeks.
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