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  • The remaining suspension was decontaminated for 15 minutes with 4 ml H2SO4 (4%) at room temperature, neutralized with 5.6 ml NaOH (1N) and buffered with 20 ml sterile phosphate buffered saline (PBS), according to document WHO/TB/98.258: Laboratory Services in Tuberculosis Control, Part III, Culture, pp. 37–42.
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