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  • After DNA denaturation for 30 min in 2M HCl at 65°C and neutralization in borate buffer pH 8.5, sections were incubated overnight at 4°C with a rat monoclonal antibody raised against BrdU (OBT-0030; Oxford Biotechnology) diluted at 1/25, and then with an Alexa-488-conjugated donkey anti-rat IgG antibody (A21208; Molecular Probes) diluted at 1/1000.
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