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A methacholine challenge test was performed according to the method of Cockcroft et al [15] and the provocative concentration of methacholine that induces a 20% fall in FEV1 (PC20) was calculated from the following standard formula [16]:where C1 = second-to-last concentration, R1 = second-to-last response, C2 = last concentration, and R2 = last response.
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