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  • PCRs were carried out in 50 µl volume containing 12.5 µl of each 2 µM primers, 1 µl of 10 mM dNTP, 10 µl of 5× reaction buffer (Promega), 0.2 µl of 5 U/µl Promega Taq DNA Polymerase and approximately 30 ng of DNA extract.
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