To evaluate the effects of repeated nicotine administration on the activity of signaling proteins (total DARPP-32, pDARPP-32 Thr34, pDARPP-32 Thr75, total CREB, and pCREB); separate two-way ANOVAs (housing condition × treatment) were performed on PFC, NAc, and striatum with environment and treatment as between-group factors.
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