PropertyValue
nif:beginIndex
  • 0 (xsd:integer)
nif:broaderContext
nif:endIndex
  • 484 (xsd:integer)
nif:isString
  • Blots were washed 10 min×3 times with wash buffer (PBS containing 0.5% Tween 20) at room temperature, and then incubated for 1 h in blocking buffer including one of the following secondary affinity-purified, horseradish peroxidase-conjugated antibodies: anti-rabbit IgG (111-035-144, Jackson ImmunoResearch, West Grove, PA; 1∶20000 for Total DARPP-32, 1∶7500 for pDARPP-Thr34 and pDARPP-Thr75), anti-mouse IgG (7076, Cell Signaling, Danvers, MA; 1∶2000 for both Total CREB and pCREB).
rdf:type