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  • PFC, nucleus accumbens (NAc) and striatum were sonicated immediately on ice in a homogenization buffer containing 20 mM HEPES, 0.5 mM EDTA, 0.1 mM EGTA, 0.4 M NaCI, 5 mM MgCI2, 20% glycerol, 1 mM PMSF, phosphatase inhibitor cocktails I (P2850, Sigma-Aldrich, St. Louis, MO) and protease inhibitors (P8340, Sigma-Aldrich, St. Louis, MO), as described previously [32].
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