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The recovery was expressed as a percentage and was calculated by subtracting the endogenous amount of analyte from the amount found, divided by the amount spiked and multiplying by 100. 2.8 Reproducibility of the HPLC-FD method: The linear relationship between analyte concentration and the area of the HPLC-FD signal was verified by three replicate analyses of three calibration standard curves (10–1000 fmols).
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