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The eluate was monitored with UV (214 nm) and fluorescence detection with the following wavelengths for excitation and emission: FA (λexcitation. 368 nm; λ emission. 524 nm) and JH III (λ ex. 450 nm; λ em. 560 nm). 2.7 Recovery efficiency for FA and JH III: The efficiency of sample recovery was investigated using two independent strategies: The addition of 100 ng of citronellol as an internal standard to the samples before derivatization with subsequent analysis of citronellol recovery using the UV detector (214 nm).
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