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  • The separation of tagged compounds was performed on an analytical column Acclaim 120 C18 (250×2.1 mm ID, particle size 5 µm) (Dionex), using isocratic elution from 0 to 20 min (acetonitrile/water, 1 to 1 v/v), followed by a linear gradient from 20 to 50 min (acetonitrile-water (50 to 95%, v/v) and another isocratic elution from 50 min (acetonitrile, 95%).
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