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Aliquot of the reactions were analyzed by HPLC-FD. 2.5.2 Analysis of insect whole body extracts: For JH III quantification, 10 adult female mosquitos (24 h after emergence) were processed by the method described by Bergot et al. [14] that includes an acetonitrile/pentane extraction and a C18 solid-phase extraction cartridge purification (Figure S2).
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