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  • For JH III quantification, 10 pairs of CA-CC complexes were dissected and incubated for 4 h in the dark at 32°C under continuous gentle agitation in tissue culture medium M-199 (Lavallette, NJ, USA) containing 2% Ficoll, 25 mM HEPES (pH 6.5) and methionine (50 µM).
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