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C) Semi-quantitative analysis of COX-2, 5-lipooxygenase (5-LOX) and p-5-LOX, and non-quantitative visualization of signaling proteins by western blotting: Three independent Western blots were conducted for each antibody for the semi-quantitative assessment of protein expression by densitometry for the AA-metabolizing enzymes COX-2 and p-5-LOX, using NIH Image J software.
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